Flow cytometry is an analysis
tool that identify, sort, and analyzes multiple physical characteristics of
cells. It is a common technique to study cellular characteristics and relations
to immune functions. This mechanism
works by suspending individual cells in a solution, allowing them to be studied
independently and simultaneously. The
properties measured include a cell’s relative size, relative granularity or
internal complexity, and relative fluorescence intensity. These characteristics
are determined using an optical-to-electronic coupling system that records how
the cell or particle scatters incident laser light and emits fluorescence. Some
flow cytometry applications include:
Flow cytometers are expensive, not only to purchase, but also to maintain so it is often difficult for a single, life science start-up to purchase one for exclusive use. Some wet lab incubators and scientific coworking spaces have flow cytometers on their equipment list such as JLabs San Diego. It is important to find out if use of specialized capital equipment is included in the monthly rent or if there is an additional charge.
If your lab does not have a flow cytometer, look to your nearest leading research university. Many have core facilities that are open to community access. These facilities offer state-of-the-art equipment and flow cytometry expertise with their staff. Some core facilities have pre-negotiated, reduced pricing for start-ups residing in their neighboring incubators.
Below is a list of five research universities in Southern California with flow cytometry core facilities open to community access including specific instruments and their listed commercial rates.
Jennifer Atwood, PhD
Vanessa Scarfone, MS
Gay Crooks, MD
Nunzio Bottini, MD, PhD
Dr. Prue Talbot
Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research at USC
1425 San Pablo Street, BCC 205B
Los Angeles, CA 90033
Jeffrey Boyd (Director)