What is Flow Cytometry?
Flow cytometry is an analysis tool that identify, sort, and analyzes multiple physical characteristics of cells. It is a common technique to study cellular characteristics and relations to immune functions. This mechanism works by suspending individual cells in a solution, allowing them to be studied independently and simultaneously. The properties measured include a cell’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. Some flow cytometry applications include:
- Cell Sorting
- Cell Cycle Analysis
- Cell Proliferation Assays
- Intracellular Calcium Flux
Where are the Flow Cytometers in Southern California?
Flow cytometers are expensive, not only to purchase, but also to maintain so it is often difficult for a single, life science start-up to purchase one for exclusive use. Some wet lab incubators and scientific coworking spaces have flow cytometers on their equipment list such as JLabs San Diego. It is important to find out if use of specialized capital equipment is included in the monthly rent or if there is an additional charge.
If your lab does not have a flow cytometer, look to your nearest leading research university. Many have core facilities that are open to community access. These facilities offer state-of-the-art equipment and flow cytometry expertise with their staff. Some core facilities have pre-negotiated, reduced pricing for start-ups residing in their neighboring incubators.
How much does it cost to use a flow cytometer at a core facility?
Below is a list of five research universities in Southern California with flow cytometry core facilities open to community access including specific instruments and their listed commercial rates.
Jennifer Atwood, PhD
Vanessa Scarfone, MS
UC Los Angeles
Gay Crooks, MD
UC San Diego
Nunzio Bottini, MD, PhD
Dr. Prue Talbot
USC Stem Cell
Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research at USC
1425 San Pablo Street, BCC 205B
Los Angeles, CA 90033
Jeffrey Boyd (Director)